Figure 8. Wg protein secretion in lqfR- clones.

Shown are confocal microscope images of two third instar larval wing discs immunostained with Wg antibodies (purple). Wingless is expressed and secreted by a stripe of cells at the dorsal/ventral boundary. Homozygous mutant clones are marked by the absence of GFP (green). (A,A′) A wing disc with vps35^E42 homozygous clones, outlined in white in A′. The genotype is hs-flp; FRT42D vps35^E42/FRT42D ubi-gfp. (B,B′) A wing disc with lqfR^Δ117 mutant clones, outlined in white in B′. The genotype is hs-flp; FRT82B lqfR^Δ117/FRT82B ubi-gfp. scale bar: ∼10 µm.