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. 2012 Sep 28;7(9):e46493. doi: 10.1371/journal.pone.0046493

Figure 2. Inhibition of Lip-HSL proteins by MmPPOX.

Figure 2

A, SDS-PAGE profile of the 9 Lip-HSL proteins used in this study, following purification using Ni2+-NTA resin. Quantity loaded: Molecular Weight (MW), 2 µg; LipC (46 kDa), 2 µg; LipF (31 kDa), 1 µg; LipH (36 kDa), 5 µg; LipI (36 kDa), 6 µg; LipN (42 kDa), 5 µg; LipR (34 kDa), 3 µg; LipU (33 kDa), 1 µg; LipW (34 kDa), 3 µg; LipY (47 kDa), 10 µg; Cut6 (31 kDa), 9 µg. B, Residual activities of LipC, LipI, LipU, LipY and Cut6 after 10 min incubation with MmPPOX at various molar excess (x I). Residual activities were measured spectrophotometrically using pNPC4 as substrate. x I50 values were defined as the inhibitor molar excess leading to 50% enzymes residual activities.