Table 1. Substrate specificity of recombinant Lip-HSL proteins.
| Substrate chain length/specific activitiesa (U/mg) | ||||||
| pNP estersb | Vinyl estersc | TAGd | ||||
| Protein | Best | Up to | Best | Up to | Best | Up to |
| LipC [18] | C4/0.12 | C10/0.02 | n.d | n.d | n.d | n.d |
| LipF | C4/0.18 | C10/0.02 | n.d | n.d | n.d | n.d |
| LipH [20] | C4/13.5 | ND | C3/1600 | C4/1100 | C3/1350 | C4/450 |
| LipI | C4/15.7 | ND | C4/73 | C4/73 | C3/30 | C4/27 |
| LipN | C4/7.2 | C14/0.04 | C4/1390 | C4/1390 | C3/350 | C4/240 |
| LipR | C8/0.53 | C12/0.27 | n.d | n.d | n.d | n.d |
| LipU | C8/0.48 | C14/0.04 | n.d | n.d | n.d | n.d |
| LipW | C4/1600 | C14/0.04 | n.d | n.d | n.d | n.d |
| LipY | C4/46.3 | C14/5.2 | C4/300 | C8/35 | C4/208 | C18:3/4.0 |
a
All activities were performed beyond the substrate solubility limit (except for pomegranate oil, which was directly coated on the plate) and 1 unit (U) corresponds to 1 µmol of fatty acid released per min.
b
Chain lengths tested include C4, C5, C8, C10, C12 and C14.
c
Chain lengths tested include C4, C6 and C8.
d
Chain lengths tested include C3, C4, C8, C18:1 (olive oil) and C18:3 (pomegranate oil).
ND not determined.
n.d not detected.