Figure 3. The transcriptional level of VE-cadherin in MSC^CXCR4 under hypoxia is mediated by STAT3 pathway.

(A): The luciferase activity of both MSC^Null and MSC^CXCR4 under normoxia or hypoxia for 24 hours was measured. (B): The VE-cadherin promoter activity of MSC^CXCR4 under hypoxia for 24 hours was impacted by various inhibitors. (C): STAT3 expression was analyzed by Western blots in MSCs under normoxia or hypoxia for 24 hours. (D): Quantitative analysis for STAT3 phosphorylation after normalization against total STAT3. (E): The VE-cadherin expression was analyzed by RT-PCR in MSC^CXCR4 treated with DMSO or WP1066 under hypoxia for 72 hours. * p<0.05; ** p<0.01. All values are expressed as mean ± SD (n = 4).