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. 2012 Sep 28;7(9):e46490. doi: 10.1371/journal.pone.0046490

Figure 2. Expression and function of Am-run.

Figure 2

Embryos are oriented with anterior left and dorsal up. Scale bars are 200 µm. (A) Expression of Am-run in a stage 4 oocyte, dorsal view. Am-run RNA is present in both nurse cells (NC) and the oocyte (O), where it is enriched at the anterior of the oocyte (arrow), and in a stripe along the oocyte surface. (B) Am-run RNA is enriched in the anterior of late stage oocytes. (C) In stage 2 embryos, Am-run RNA is present in a faint broad domain in central regions of the embryos, marked by arrowheads in C. (D) Mid stage 4 embryo with stripes of cells expressing Am-run RNA appearing across the anterior–posterior axis. (E) By gastrulation (stage 6) distinct stripes of cell expressing Am-run mRNA are detected throughout thoracic and abdominal regions. These stripes of cells often display darker staining in cells at the anterior of each stripe and then split as stage 6 progresses. (F) Am-run RNAi larva, showing disrupted thoracic and abdominal segments. Segments appear more widely spaced than in controls, as implied by the pattern of trachea, perhaps indicating loss of alternate segments. Larvae also show expansion of the labrum (lb) and loss of head appendages (gnathum). Asterisks mark segmental trachea.