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. 2012 Sep 28;7(9):e46462. doi: 10.1371/journal.pone.0046462

Figure 2. The Collagen-like tandem repeat and the C-terminal domains of Lcl are essential for biofilm formation.

Figure 2

(A) Anti-Lcl immunoblot analyses against Lp02 and Lp02 Δlpg2644 lysates untransformed and transformed with plpg2644, plpg2644 Δrepeat and plpg2644 ΔC-terminal. The positions of the molecular size markers are indicated on the left of the blot in kDa. (B) Static biofilm assays with Lp02 and Lp02 Δlpg2644 producing recombinant variants of Lcl (plpg2644 Δrepeat and plpg2644 ΔC-terminal) and full length recombinant Lcl (plpg2644). * Two-tailed Student’s t-test P-value <0.001 versus Lp02).