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Total bacteria were labelled with SYTO62 and one strain of each 1∶1 mixture was labelled with GFP. Confocal scanning laser microscopy (CSLM) micrographs show the distribution of L. pneumophila within static biofilms using (A) GFP- Lp02 Δlpg2644/Lp02, (B) GFP-Lp02/Lp02, (C) GFP- Lp02 Δlpg2644/Lp02 Δlpg2644, (D) GFP- Lp02/Lp02 Δlpg2644. (Magnification 100 X).
