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. 2012 Sep 28;7(9):e46402. doi: 10.1371/journal.pone.0046402

Figure 6. Influence of CadF on C. jejuni adhesion to inert surfaces.

Figure 6

(A) Dot blotting using anti-CadF antibodies of 0.5, 1, 5 and 10 µg of OMP-enriched fraction of C. jejuni 81–176 (WT) and the derivative mutant C. jejuni 81–176 ΔcadF. Ctl is the control without protein. (B) Silver-stain two-dimensional electrophoretic gel of the OMP-enriched fraction of oxygen-acclimated cells and the corresponding immunoblot using anti-CadF antibodies. (C) Adhesion capability after 2 h of oxygen-acclimated cells and paraquat-stressed cells of C. jejuni 81–176 mutant ΔCadF (CadF−) and its isogenic strain. Initial bacterial concentration anged from 8.72 to 8.85 Log(CFU/mL). Error bars represent the standard deviation of three independent assays. Asterisks indicate significant differences in comparison with the isogenic strain (P<0.05).