Table 2. Identification and localization prediction (PSORTb v3.0.2) of proteins predominantly modulated by oxygen-acclimated conditions in the IMP and OMP enriched fractions of C. jejuni.
| Accession number | Protein ID | Prot/Spot | P-value* | n-Fold | pI/MW | Mascot score | NMP/Pc | Fraction localization | Cell localization prediction |
| Transporters | |||||||||
| gi|121612178 | CjaA protein | CjaA-1 | 0.003 | up2.0 | 5.69/30949 | 799 | 29/66% | IM | PP/IM |
| putative amino acid | CjaA-2 | 0.009 | up2.2 | 5.69/30949 | 699 | 22/65% | IM | PP/IM | |
| transporter | CjaA-3 | 0.002 | up1.9 | 5.69/30949 | 339 | 14/55% | OM | PP/IM | |
| [surface antigen CjaA] | CjaA-4 | 0.008 | up2.4 | 5.69/30949 | 358 | 13/54% | OM | PP/IM | |
| CjaA-5 | 0.006 | up1.6 | 5.69/30949 | 865 | 32/75% | OM | PP/IM | ||
| CjaA-6 | 0.005 | up2.0 | 5.69/30949 | 485 | 18/55% | OM | PPIM | ||
| gi|121612379 | histidine-binding protein HisJ (surface antigen CjaC) CjaC protein | CjaC | do2.4 | 6.48/27781 | 376 | 10/34% | OM | PP | |
| gi|121613528 | high affinity branched-chain amino acid ABC transporter, ATP-binding protein | LivF | 0.002 | do2.3 | 7.03/25739 | 510 | 16/58% | IM | CytP |
| gi|121612467 | outer membrane lipoprotein CmeC RND efflux system, | CmeC-1 | 0.01 | do1.7 | 5.14/55385 | 598 | 13/29% | OM | OM |
| CmeC-2 | 0.003 | do1.9 | 5.14/55385 | 526 | 12/27% | OM | OM | ||
| Chaperones | |||||||||
| gi|121612249 | chaperonin GroEL | GroEL-1 | 0.0001 | up3.3 | 5.02/57934 | 945 | 27/47% | IM | CytP |
| GroEL-2 | 0.007 | up1.6 | 5.02/57934 | 2350 | 68/44% | IM | CytP | ||
| GroEL-3 | 0.005 | up1.8 | 5.02/57934 | 1724 | 45/55% | IM | CytP | ||
| gi|121612930 | co-chaperonin GroES | GroES | 0.004 | do2.2 | 5.38/9452 | 348 | 12/87% | IM | CytP |
| gi|121613084 | molecular chaperone DnaK | DnaK | 0.005 | do2.5 | 4.98/67403 | 206 | 6/12% | OM | CytP |
| gi|121612573 | co-chaperone protein DnaJ | DnaJ1 | 0.003 | up9.0 | 8.86/33320 | 79 | 3/10% | IM | CytP |
| gi|121613623 | ATP-dependent chaperone protein ClpB | ClpB-1 | 0.004 | do3.5 | 5.47/95489 | 1802 | 56/56% | IM | CytP |
| ClpB-2 | 0.003 | do2.4 | 5.47/95489 | 1742 | 56/52% | IM | CytP | ||
| ClpB-3 | 0.002 | do2.0 | 5.47/95489 | 607 | 19/22% | IM | CytP | ||
| ClpB-4 | 0.006 | do2.4 | 5.47/95489 | 2692 | 88/68% | IM | CytP | ||
| Fatty acid biosynthesis | |||||||||
| gi|121612451 | biotin carboxylase | AccC_2-1 | 0.00001 | do1.8 | 6.01/49116 | 191 | 7/19% | IM | CytP |
| AccC_2-2 | 0.001 | do2.8 | 6.01/49116 | 165 | 5/17% | IM | CytP | ||
| gi|121613559 | putative lipoprotein | CJJ_0430 | 0.01 | up3.3 | 5.29/33188 | 254 | 9/29% | OM | unknown |
| Adhesion/virulence | |||||||||
| gi|121612545 | flagellin | FlaA-1 | 0.00003 | up2.1 | 5.61/59507 | 1518 | 41/43% | IM | EC |
| FlaA-2 | 0.00006 | up2.0 | 5.61/59507 | 1180 | 29/43% | IM | EC | ||
| FlaA-3 | 0.0003 | up2.0 | 5.61/59507 | 1694 | 45/48% | IM | EC | ||
| FlaA-4 | 0.0007 | up1.8 | 5.61/59507 | 1131 | 31/45% | IM | EC | ||
| FlaA-5 | 0.00001 | up2.1 | 5.61/59507 | 1426 | 34/40% | IM | EC | ||
| FlaA-6 | 0.00013 | up1.8 | 5.61/59507 | 1383 | 33/46% | OM | EC | ||
| FlaA-7 | 0.0004 | up2.7 | 5.61/59507 | 601 | 15/36% | OM | EC | ||
| FlaA-8 | 0.0007 | up1.9 | 5.61/59507 | 1884 | 53/57% | OM | EC | ||
| FlaA-9 | 0.002 | up2.3 | 5.61/59507 | 253 | 10/24% | OM | EC | ||
| FlaA-10 | 0.003 | up2.0 | 5.61/59507 | 402 | 19/33% | OM | EC | ||
| FlaA-11 | 0.001 | up2.4 | 5.61/59507 | 2379 | 67/60% | OM | EC | ||
| FlaA-12 | 0.0003 | up3.3 | 5.61/59507 | 1999 | 46/53% | OM | EC | ||
| gi|121613214 | flagellar hook protein FlgE | FlgE-1 | 0.005 | up1.7 | 5.14/89392 | 915 | 30/39% | OM | EC |
| FlgE-2 | 0.0006 | up1.6 | 5.14/89392 | 1320 | 36/37% | OM | EC | ||
| gi|121613274 | chemotaxis histidine kinase CheA | CheA-1 | 0.005 | up2.4 | 4.88/85168 | 889 | 20/28% | IM | CytP |
| CheA-2 | 0.006 | up2.2 | 4.88/85168 | 370 | 11/15% | IM | CytP | ||
| CheA-3 | 0.0006 | up2.7 | 4.88/85168 | 451 | 13/19% | IM | CytP | ||
| gi|121612668 | major outer membrane protein (MOMP) | PorA-1 | 0.002 | up2.9 | 4.72/45681 | 281 | 8/20% | IM | OM |
| PorA-2 | 0.002 | up1.7 | 4.72/45681 | 616 | 14/38% | IM | OM | ||
| gi|121612905 | cell-binding factor 2 major antigenic peptide Peb4 CBF2 | Cbf2 (Peb4) | 0.00003 | up5.1 | 9.23/30411 | 479 | 19/51% | IM | PP |
| gi|121612147 | fibronectin-binding protein | CadF-1 | 0.004 | up1.7 | 5.89/35967 | 202 | 6/12% | OM | OM |
| CadF-2 | 0.005 | up1.6 | 5.89/35967 | 540 | 13/34% | OM | OM | ||
| CadF-3 | 0.006 | do1.8 | 5.89/35967 | 235 | 6/16% | OM | OM | ||
| gi|121613534 | fibronectin type III domain-containing protein | CJJ_1295 | 0.0007 | up1.6 | 5.91/46079 | 511 | 19/42% | OM | unknown |
| Other | |||||||||
| gi|121612430 | elongation factor Tu | Tuf-1 | 0.005 | up1.7 | 5.11/43566 | 918 | 28/48% | IM | CytP |
| Tuf-2 | 0.009 | up1.6 | 5.11/43566 | 2361 | 65/75% | IM | CytP | ||
| Tuf-3 | 0.004 | do2.2 | 5.11/43566 | 570 | 15/36% | IM | CytP | ||
| Tuf-4 | 0.001 | up1.7 | 5.11/43566 | 992 | 31/68% | OM | CytP | ||
| gi|121613042 | serine protease DO | HtrA-1 | 0.006 | up1.6 | 8.97/50985 | 1515 | 44/61% | IM | PP |
| HtrA-2 | 0.009 | up1.7 | 8.97/50985 | 1626 | 50/62% | IM | PP | ||
| HtrA-3 | 0.00007 | up4.3 | 8.97/50985 | 475 | 15/31% | IM | PP | ||
| gi|121613659 | malate dehydrogenase | Mdh | 0.002 | up2.0 | 5.46/33379 | 212 | 5/19% | IM | CytP |
| gi|121612371 | succinyl-CoA synthase, beta subunit | SucC | 0.006 | do1.7 | 5.61/41716 | 164 | 6/16% | IM | CytP |
| gi|121612541 | isocitrate dehydrogenase, NADP-dependent | Icd-1 | 0.004 | do2.9 | 6.85/86316 | 156 | 5/9% | IM | CytP |
| Icd-2 | 0.007 | do2.1 | 6.85/86316 | 954 | 27/36% | IM | CytP | ||
| gi|121612912 | pyruvate kinase | Pyk | 0.005 | do1.8 | 5.89/53751 | 485 | 16/31% | IM | CytP |
| gi|121612884 | arylsulfate sulfotransferase, degenerate | CJJ_0882 | 0.005 | do1.8 | 7.57/69255 | 411 | 15/27% | IM | unknown |
| gi|121613032 | mur ligase family protein | CJJ_0816 | 0.01 | do2.3 | 9.22/55168 | 34 | 2/4% | OM | unknown |
| gi|121613329 | tyrosyl-tRNA synthetase | TyrS | 0.0005 | do3.4 | 6.31/45347 | 31 | 5/16% | OM | CytP |
| gi|121613150 | hypothetical protein CJJ81176_1382 | CJJ_1382 | 0.00005 | up3.5 | 8.84/26552 | 143 | 4/23% | IM | OM/PP/EC |
| gi|121613455 | hypothetical protein CJJ81176_0729 | CJJ_0729 | 0.006 | up1.6 | 5.60/27722 | 795 | 23/67% | IM | CytP |
| gi|121613526 | 7-alpha-hydroxysteroid dehydrogenase | CJJ_0828 | 0.001 | up1.8 | 6.77/28119 | 1025 | 35/88% | IM | CytP |
| gi|121612484 | hypothetical protein CJJ81176_0854 | CJJ_0854 | 0.007 | up7.3 | 5.70/36925 | 16 | 3/11% | IM | CytP |
| gi|121612647 | hypothetical protein CJJ81176_0275 | CJJ_0275 | 0.002 | up7.8 | 5.32/31916 | 16 | 3/13% | IM | unknown |
*
Only spots with a q-value (False Discovery Rate) <0.05 and a P (Power)>0.8 were conserved.
Spot refers to spots detected from 2-DE gel analysis (Fig. 2A and B), N-fold: protein abundance difference between 5% O2 and 19% O2, up: higher abundance protein, do: lower abundance protein, pI: protein isoelectric point; MW: protein molecular weight (Da); NMP: number of matching peptides, Pc: % of protein coverage, OM: outer membrane, IM: inner membrane; PP: periplasm, EC: extracellular, CytP: cytosol. The identification of the proteins indicated in italic was not statistically validated.