Figure 4. Ca²⁺ induced swelling in isolated mitochondria.

(A) Representative traces of 100–1000 µmol/L Ca²⁺ induced a decrease in light transmission in the ischemia/reperfusion (I/R) 24 h group. (B) Graph showing that the Ca²⁺-induced reduction in light transmission was dose dependent at concentrations of 100–1000 µmol/L (compared with control or compared with each other between the different concentrations of calcium; in each group, there was a statistically significant difference). This reduction gradually increased with the extension of reperfusion time from 2 hours to 72 hours (from 100 to 1000 µmol/L Ca²⁺, compared with each other between the three reperfusion groups, there was a statistically significant difference). The control in B indicates without Ca²⁺ intervention. (C) Representative traces of 200 µmol/L Ca²⁺ induced a decrease in light transmission in the sham group, the ischemia (I) 2 h group and the ischemia/reperfusion (I/R) 2 h, I/R 24 h and I/R 72 h groups. (D) A graph showing that the 200 µmol/L Ca²⁺-induced reduction in light transmission was increased with the extension of the reperfusion time from 2 hours to 72 hours. Six animals were included in each group. Each bar represents the means±SD. ^#P<0.05 versus sham; ^*P<0.05 versus I 2 h, I/R 2 h and sham; ^⋇P<0.05 versus I 2 h, I/R 2 h, I/R 24 h and sham.