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. 2012 Sep 28;7(9):e46658. doi: 10.1371/journal.pone.0046658

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© 2012 Crombach et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Screenshot displaying the ‘Mask Details’ tab of our FlyGUI. This tab is used for embryo re-orientation, staging, and quality control for the segmentation process. Different embryo images are displayed by clicking on the curved direction arrows (shown in A–E). (A,B) Intermediate images generated after the ‘edge detection’ and ‘fill holes’ operations in the processing pipeline. (C–E) Cropped and rotated DIC, bright-field, and nuclear counterstain images. (F) Pop-out high-resolution image of membrane morphology. See text for details.