Table 3. Effect of DGPP 10 µM on FAAH activity and expression.
FAAH activity | FAAH expression | ||
nmoles [3H]-AA/mg prot/h | FAAH mRNA/actin | FAAH protein/actin | |
control | 1,2±0,2 | 1,0±0,0 | 1,0±0,0 |
DGPP 10 µM | 1,1±0,2 | 1,1±0,2 | 1,2±0,1 |
Uterine strips from rats pregnant on day 5 of gestation were incubated for 6 h with DGPP 10 µM. FAAH activity was determine by radioconversion and FAAH mRNA and protein expression was analyzed by RT-PCR and Western Blot respectively.