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. 2012 Sep 28;7(9):e44911. doi: 10.1371/journal.pone.0044911

Figure 6. Saturation binding curve of NK1R-NLPs to FAM-SP.

Figure 6

[NK1R-NLPs] is the concentration of free NK1R-NLPs at equilibrium and was calculated from the subtraction of the amount of NK1R-NLPs bound with FAM-SP from the total amount of NK1R-NLPs added. The ligand bound % was calculated by comparing the average diffusion time of the mixture (free FAM-SP and FAM-SP bound with NK1R-NLPs) with the individual controls (diffusion times of free FAM-SP and free NK1R-NLPs). The experimental data (blue dots) were fitted to an “OneSiteBind” model Y  =  Bmax × X/(Kd + X). Y represents the percentage of bound ligand in the total amount of ligand, and X represents the concentration of NK1R-NLPs in the solution after reaction. The fitting results in 36±5.6 nM for Bmax and 83±33 nM for Kd.