Figure 3. The aCGH results were validated by qPCR using the flow-sorted DNA.

Primers were designed for PKP4 and pre-miR-651 genomic sequences. Primers were also designed using sequences outside the deleted regions for PKP4 and pre-miR-651 and were called PKP4-control and pre-miR-651-control respectively. Actin was used as the universal control. qPCR was performed and fold change was calculated and plotted. The left lower lung tumor sample validated as expected for pre-miR-651 and PKP4 deletion and the brain right frontal lobe sample did not show any of these deletions validating the aCGH results. The jejunum sample did not validate for these results.