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. 2012 Sep 28;7(9):e45172. doi: 10.1371/journal.pone.0045172

Figure 1. Functional requirement for dynamin of FMDV and VSV infection.

Figure 1

(A) BHK-21 cells transfected with eGFP fused to WT or a DN version of dynamin (eGFP-Dyn WT and eGFP-Dyn K44A, respectively) and 24 h later were incubated with the different FMDV variants (C-S8c1 and MARLS) or VSV (MOI of 70 PFU/cell) for 25 min and processed for immunofluorescence. Nuclei were stained using ToPro-3 (blue). GFP and viruses are shown in green and red, respectively. Bar: 10 µm. (B) BHK-21 cells transfected and infected as in (A). The graph represents the percentage of cells that showed internalized virus, determined as described in Materials and Methods. At least 100 transfected cells per coverslip were scored in each assay (3 coverslip). (C) BHK-21 cells were electroporated with a plasmid encoding eGFP-Dyn WT as control, or eGFP-Dyn K44A. At 24 h post-electroporation, monolayers were infected with the corresponding virus (MOI of 1 PFU/cell). Cells were fixed and processed for immunofluorescence at 7 h post-infection. Bars represent the mean percentage of transfected and infected cells ± SD, normalized to the level of infection of cells expressing the eGFP-Dyn WT. Statistically significant differences between cells transfected with eGFP-Dyn WT or K44A are indicated by an asterisk (ANOVA P≤0.05).