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. 2012 Sep 28;7(9):e46083. doi: 10.1371/journal.pone.0046083

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–C), Relative expression of various components of Shh pathway was quantified in human pancreatic cancer stem cells (PanCSC), human pancreatic normal stem cells (HNPSC) and human pancreatic normal ductal epithelial cells (HPNE). The expression of Shh genes was quantified using quantitative reverse transcriptase polymerase chain reaction real-time assay (q-RT-PCR), and normalized to GAPDH expression. All assays were performed in triplicate and were calculated on the basis of ΔΔCt method. Data represent mean ± SD. %, and $ = significantly different from control, P < 0.05. (D), Immunoblotting of Gli1/2, Smo and β-actin of human pancreatic cancer stem cells (PanCSC), human pancreatic normal stem cells (HNPSC) and human pancreatic normal ductal epithelial cells (HPNE).