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. 2012 Sep 28;7(9):e46083. doi: 10.1371/journal.pone.0046083

Figure 8. Regulation of Bcl-2 expression, caspase-3/7 activity, and apoptosis by SFN on Pancreatic CSCs.

Figure 8

(A), Effects of SFN on BcL-2expression. q-RT-PCR was performed to examine the expression of BcL-2. All assays were performed in triplicate and were calculated on the basis of ΔΔCt method. Data represent mean ± SD. @, % and $ = significantly different from control, P < 0.05. (B), Effects of SFN on caspase-3/7 activity. Pancreatic CSCs treated with SFN (0–20 µM) for 24 h, and caspase-3/7 activity was measured as per manufacturer's instructions. Data represent mean ± SD. @, %, and $ = significantly different from control, P < 0.05. (C), Pancreatic CSCs were treated with SFN (0–20 µM), and cell lysates were collected and Immunobloted for anti- BCL-2, cleaved Caspase 3 or β-actin antibody. (D), Effects of SFN on apoptosis. Pancreatic CSCs were treated with SFN (0–20 µM) for 48 h, and apoptosis was measured by PI staining using flow cytometry.