Table 2. Information used for the synthesis reactions of E. coli’s metabolic genes.

Information	Subsystem/Reaction	Source/Reference
Transcription unit	Transcription	EcoCyc [60]
Gene coordinate, direction	Transcription	Riley et al. [61]
Gene function	Metabolism	iAF1260 [5]
Protein information	Protein complex formation	iAF1260 [5], EcoCyc [60], primary literature
Metallo-ion	Metallo-ion binding	EcoCyc [60], protein structure, primary literature
Prosthetic group	Protein complex formation	EcoCyc [60], protein structure, primary literature