Table 2. High grade prostate cancer (Gleason score 8 and above) biomarker gene list and differential expression ratio in Cohort II verification sample set (80 disease and 102 controls).

Gene Name#	Description	Sequence Accession ID	Expression Fold Change†	Expression P Value‡	Expression AUC*
CRTAM	cytotoxic and regulatory T cell molecule	NM_019604	1.58	3.46E-05	0.67
CXCR3	chemokine (C-X-C motif) receptor 3	NM_001504	1.59	3.38E-05	0.66
FCRL3	Fc receptor-like 3	NM_052939	1.61	2.85E-06	0.69
KIAA1143	KIAA1143	NM_020696	1.44	1.82E-07	0.73
KLF12	Kruppel-like factor 12	NM_007249; NM_016285	1.66	8.16E-07	0.71
TMEM204	transmembrane protein 204	NM_024600	1.52	8.40E-05	0.67
SAMSN1	SAM domain, SH3 domain and nuclear localization signals 1	NM_022136	–	–	–

# The 7 biomarkers were picked up from the 10 that were verified in Cohort II samples, using gene-ratio algorithm, based on the best AUC of combined gene-pair.

^†Determined by qRT-PCR analysis using SAMSN1 as a partner gene, gene ratio was calculated using delta delta Ct calculation.

^‡Calculated by Mann-Whitney test.

^*area under receiver-operating-characteristic curve.