Table 1. BALF Cytology.
| 4-Week Air Exposure | ||||||
| Total cells | AM | Neutrophils | Lymphocytes | Eosinophils | ||
| Saline | 2.18±0.68 | 2.03±0.69 | 0.03±0.01 | 0.04±0.01 | 0.08±0.03 | |
| % | 89.4±3.5 | 1.8±0.6 | 3.3±1.2 | 5.5±2.6 | ||
| CNP | 2.25±0.13 | 1.43±0.14 | 0.73±0.08a | 0.08±0.01 | 0.01±0.00 | |
| % | 63.4±4.1a | 32.6±3.7a | 3.4±0.3 | 0.6±0.2 | ||
| OVA | 2.60±0.67 | 1.21±0.28 | 0.16±0.08b | 0.08±0.02 | 1.16±0.37a,b | |
| % | 47.0±1.7a,b | 6.4±2.2b | 3.0±0.5 | 43.5±2.9a,b | ||
| OVA+CNP | 2.18±0.30 | 1.17±0.21 | 0.15±0.05b | 0.22±0.04a,b,c | 0.84±0.11 | |
| % | 48.3±4.1a,b | 6.1±1.7 b | 9.4±2.1a,b,c | 36.2±4.2a,b,c | ||
| 4-Week NO2 Exposure | ||||||
| Total cells | AM | Neutrophils | Lymphocytes | Eosinophils | ||
| Saline | 0.75±0.11 | 0.63±0.1 | 0.05±0.01 | 0.03±0.01 | 0.05±0.01 | |
| % | 82.9±2.0 | 6.1±0.6 | 4.4±0.8 | 6.6±1.6 | ||
| CNP | 2.82±1.57 | 2.08±1.16a | 0.63±0.36a | 0.08±0.03 | 0.03±0.01 | |
| % | 72.7±1.9 | 22.5±1.9a,d | 3.3±0.4 | 1.5±0.5 | ||
| OVA | 3.94±0.56 | 1.79±0.23 | 0.11±0.04b | 0.17±0.04a,b,d | 1.86±0.41a,b | |
| % | 46.8±4.1a,b | 3.2±1.0b | 4.8±1.1 | 45.3±5.0a,b | ||
| OVA+CNP | 3.47±0.71 | 1.38±0.33 | 0.08±0.02b | 0.14±0.01a,d | 1.87±0.46a,b,d | |
| % | 40.2±4.3a,b | 3.5±1.5 b | 4.8±0.9d | 51.6±5,4a,b,d | ||
Values are means ± SE (n = 6 per group);
a
p<0.05 vs. Saline within condition;
b
p<0.05 vs. CNP within condition;
c
p<0.05 vs. OVA within condition;
d
p<0.05 vs. Air within treatment, by two way ANOVA; AM: alveolar macrophages. Exposure to NO2 alone did not change the profile of BAL cytology. OVA-sensitization significantly increased BAL eosinophil counts. CNP exposure by itself increased the number of neutrophils, without a significant difference between Air and NO2-exposed animals. Exposure to NO2 in OVA-sensitized animals significantly increased lymphocyte counts and tended to elevate BALF eosinophilia.