Figure 3. TLR7 ligand treatment reduced clinical severity, and T cell responses of EAE mice.
(A) SJL/J mice were i.p. immunized with PLP139–151 in CFA with Ptx. Mice (n = 26/group) received 150 nmol/animal 1V136 or vehicle s.c. daily from day 6 onward. The mice were scored for disease and the data were pooled from four independent experiments. Bar indicates p<0.05 by two-way ANOVA with Bonferroni post hoc tests. (B) SJL mice were immunized as above (n = 14/group) and treated from day 5 to 18 with 150 nmol/animal 1V136 or vehicle s.c. and disease was monitored up to 60 days. Data are pooled from 2 independent experiments and the bar indicates p<0.05 by two-way ANOVA with Bonferroni post hoc tests. (C, D) Splenocytes obtained from vehicle- (n = 5) and 1V136-treated (n = 5) SJL/J EAE mice at 19 days were restimulated in vitro with PLP139–151 peptide for 3 days. Levels of IFN-γ (C), and IL-17 (D) in the supernatants were determined by ELISA. Data shown are means ± SEM and are representative of three independent experiments that showed similar trends. p<0.05 compared vehicle-treated group by Student t test.