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. 2012 Sep 28;7(9):e45836. doi: 10.1371/journal.pone.0045836

Figure 4. Crm1-Mst1-Aurora B kinase axis dictates the maintenance of stable kinetochore-microtubule attachments.

Figure 4

A) Mitotic tsBN2 cells were immunostained with anti-Crm1 and anti-Mst1 following incubation at permissive or non-permissive temperature. B) Magnified images of the boxed regions illustrating anti-Crm1 and anti-Mst1 staining (magnified merged image is exclusive of DNA). C) Quantified Crm1 and Mst1 intensities were normalized and presented as relative fold change ± s.d. (error bar) of three independent experiments. D) Co-immunoprecipitation assay was conducted using monoclonal anti-Mst1 antibody on mitotic tsBN2 cell lysates harvested 4 hours after incubation at permissive or non-permissive temperature. E) Quantified Crm1 intensities were normalized and presented as relative fold change ± s.d. (error bar) of three independent experiments. F) Time-lapse imaging of metaphase tsBN2 cells expressing H2B-GFP and Mst1 WT-mCherry, Mst1 K59R-mCherry or mCherry (positive control). Overexpression of Mst1 WT abrogated the misalignment phenotype in cells incubated at non-permissive temperature. Arrows pointed to misaligned chromosomes. Scale bar: 10 µm.