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Selected 1H NMR spectra of 1 mM L-aspartate a) before and b)–e) 10–80 min after addition of 3 µM ADC in D2O at 25°C. The diminishing signals of L-aspartate and the emerging ones of those corresponding to β-alanine permitted a direct monitoring of the enzymatic transformation and integration of the proton signals allowed for a kinetic profiling of the reaction (cf. Fig. 3).
