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. 2012 Sep 28;7(9):e46101. doi: 10.1371/journal.pone.0046101

Figure 4. Bone marrow erythroblast subsets.

Figure 4

Fresh bone marrow cell suspensions from the tibia of naive, bled, P.c. adami-infected and PHZ-treated female BALB/c mice (4–6 weeks old) were stained with anti-CD71-FITC and anti-Ter119-PE antibody for analysis of stage specific erythroblasts. Ter119+ cells were gated in R1 whereas a R2 region selected proerythroblasts (A). Cells within the R1 gate (Ter119 high) were further analyzed with respect to their CD71 and forward scatter (FSC) profiles (B) and the percentages of proerythroblasts (C), basophilic erythroblasts (R3) (D), basophilic/polychromatic erythroblasts (R4) (E) and late polychromatophilic erythroblasts (R5) (F) were estimated. Data are mean ± SEM from n = 4–5 mice per group and are compared to naive mice (*P<0.05, **P<0.01, ***P<0.001), bled mice (ε P<0.01, εεP<0.01, εεεP<0.001) or between Plasmodium-infected and PHZ-treated mice (ΨΨΨ P<0.001) using a one– way ANOVA and Tukey's multiple test.