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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Neurobiol Dis. 2012 Jul 17;48(3):399–408. doi: 10.1016/j.nbd.2012.07.011

Fig. 6.

Fig. 6

Switching from the normal HCO3−/CO2-buffered saline to HEPES-buffered saline alters the pattern of stimulation-induced changes in YFP fluorescence, especially in terminals from symptomatic mice. Each panel superimposes stimulation-induced changes in YFP fluorescence recorded in HCO3−/CO2- and HEPES-buffered saline, averaged for hSOD1-wt, presymptomatic hSOD1-G85R, and symptomatic hSOD1-G85R terminals. All terminals acidified more in HEPES than in bicarbonate buffer; this difference was especially marked in symptomatic hSOD1-G85R terminals. hSOD1-wt data came from 226 terminals in 5 animals (bicarbonate) and 211 terminals in 4 animals (HEPES). Comparable numbers for presymptomatic hSOD1-G85R: 186/4 bicarb, 220/4 HEPES; for symptomatic hSOD1-G85R: 134/6 bicarb, 282/7 HEPES.