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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Nat Methods. 2012 Sep 9;9(10):1005–1012. doi: 10.1038/nmeth.2171

Figure 1.

Figure 1

Assessment of FRET reporter performance using Förster equations and emission spectra. (a) Normalized spectra of ECFP and EYFP. Variants of CFP and YFP have identical spectra. (b-d) Modeling of existing FRET reporter performance using Förster equations and emission spectra. Ranges for the FRET efficiency E were derived by fitting theoretical to experimentally-obtained emission curves (left) and were then mapped to appropriate Förster E-r curves (right) for Camuiα (b), AKAR2 (c), or Raichu-RhoA (d). (e) Clover emission and mRuby2 excitation spectra show substantial overlap while Clover and mRuby2 emission spectra are better separated than CFP and YFP emission spectra.