Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Nat Methods. 2012 Sep 9;9(10):1005–1012. doi: 10.1038/nmeth.2171

Figure 4.

Figure 4

Reporting of fast local RhoA activation in neurons with Raichu-RhoA-CR. (a) Design of Raichu-RhoA-CR, based on Raichu-RhoA. (b) Ephrin-A stimulation locally activates RhoA in a hippocampal growth cone (asterisk) from the first time point after stimulation. Scale bar is 10 μm. (c) Raichu-RhoA-CR emission ratio graphed as mean ± s.e.m. (n = 5 cells). Peak ratio change (asterisk) was significantly different from baseline by two-tailed t-test (P = 0.0187). (d) With Raichu-RhoA, peak ratio change was not significantly different from baseline (P = 0.468, n = 8 cells).