. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Nat Methods. 2012 Sep 9;9(10):1005–1012. doi: 10.1038/nmeth.2171

Table 2.

Examples of r₀ values of fluorescent protein FRET pairs

FRET pair	ϕ _D ^a	ε_A^b (mM^–1 cm^–1)	r₀ (nm)
ECFP-Citrine	0.36^c	77^d	4.8
ECFP-Venus	0.36^c	92^d	5.0
Cerulean-Citrine	0.49^c	77^d	5.4
Cerulean-Venus	0.49^c	92^d	5.2
SECFP-SEYFP	0.58^e	101^e	5.4
EGFP-mCherry	0.60^d	72^d	5.4
TagGFP-TagRFP	0.59^f	100^f	5.7
mTFP1-Citrine	0.85^d	77^d	5.7
mTFP1-mOrange	0.85^d	71^d	5.7
Citrine-mKate2	0.76^d	63^d	5.8
Clover-mCherry	0.76^g	72^d	5.8
mTurquoise1-SEYFP	0.84^c	101^e	5.8
mTurquoise2-SEYFP	0.93^c	101^e	5.9
Clover-mRuby2	0.76^g	113^g	6.3

Random interfluorophore orientation is assumed. Pairs are ordered by r₀.

Quantum yield of donor.

Extinction coefficient of acceptor.

Values from ref. 29.

Values from ref. 45.

Values from ref. 48.

Values from ref. 15.

Data from this study.