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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Nature. 2012 Sep 19;489(7417):513–518. doi: 10.1038/nature11514

Figure 4. New rnk-citT module confers Cit+ phenotype in potentiated background.

Figure 4

a. Engineered construct containing the cit amplification junction with the rnk promoter, and the arrangement following its insertion into the chromosome. b. Average growth trajectories in DM25 of potentiated clone ZDB30 (red), its isogenic construct with chromosomal integration of the rnk-cit module ZDB595 (blue), 31,500-generation Cit+ clone ZDB564 (purple), and 32,000-generation Cit+ clone ZDB172 (green). c. Trajectories of ZDB595 compared to its parent. Light blue trajectories show heterogeneity among replicate cultures of ZDB595; dark blue and red are averages for ZDB595 and its parent, ZDB30, as in panel b (except different scale).