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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Nature. 2012 Sep 19;489(7417):513–518. doi: 10.1038/nature11514

Figure 5. Refinement of Cit+ phenotype by increased number of rnk-citT modules.

Figure 5

a. Change in rnk-citT module copy number in sequenced Cit+ clones over time. b. Structure of rnk-citT module cloned into high-copy plasmid pUC19 to produce pZBrnk-citT. c. Growth trajectories in DM25 of potentiated Cit− clone ZDB30 (red), 31,500-generation Cit+ clone ZDB564 (purple), 33,000-generation Cit+ clone CZB152 (black), and ZDB612, a pZBrnk-citT transformant of ZDB30 (green). Each trajectory is the average of six replicates.