Figure 7. Mutations that produced Cit⁺ phenotype in 14 replay experiments.

The red box shows the boundaries of the 2,933-bp amplified segment that actualized the Cit⁺ function in the original long-term population. Blue boxes show citT-containing regions amplified in 8 replays that produced Cit⁺ mutants. Vertical black lines mark 5 locations where IS3 insertions produced Cit⁺ mutants in 6 other replays.