Figure 4.

Depletion of CD4⁺ T cells abrogates protection from sand fly–transmitted Leishmania in rLJM11-immunized mice. Mice were immunized with rLJM11 or BSA in the left ear. Forty-eight hours before infected challenge and weekly thereafter, rLJM11-immunized mice were treated with anti-CD4 (GK 1.5) or isotype (IgG2b). (a) CD4⁺ and CD8⁺ T cells in peripheral blood 48 hours and 1 week following challenge with infected sand flies. The frequency of cells is indicated on the corners of contour plots. (b) Lesion size of animals immunized with rLJM11 and treated with anti-CD4 (▴) or isotype (▪) and in BSA-immunized mice (•). ^*P<0.05; ^**P<0.01; ^***P<0.001. Representative (a) and cumulative (b) data are shown (n=5).