Figure 4.

The targeting efficacy of the induction of LcrV-specific pulmonary cellular immunity. BALB/c were primed and boosted i.n. with different forms of LcrV protein of various dosages as shown at the bottom of the graphs. Six to 10 days later, lung cells were collected, restimulated, and IFN-γ or IL-2 or TNF-α secreting CD4⁺ T cells were assessed by ICS. Data are mean values from 2 independent experiments, using 2 mice per group. **p<0.005; ***p<0.001