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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Exp Eye Res. 2012 Jun 17;102:28–37. doi: 10.1016/j.exer.2012.06.004

Figure 2.

Figure 2

Immunostaining of two month old mouse lenses with an antibody raised against the C-terminus of Cx50. Cx50 is strongly localized to the lens fiber cell membranes in WT lenses (A) while Cx50 protein is less sharply localized in homozygous Cx50 G22R (D) and S50P (G) mutants. Further, homozygous Cx50G22R (D) and Cx50S50P (G) lenses also exhibit appreciable levels of Cx50 protein surrounding the nuclear envelope (arrows). Red-Cx50; Blue-DRAQ5 (DNA); e-epithelial cells; f- fiber cells; tz-transition zone; Scale bar=77μm