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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Exp Eye Res. 2012 Jun 17;102:28–37. doi: 10.1016/j.exer.2012.06.004

Figure 9.

Figure 9

Western blotting analysis of XBP1, ATF4 and ATF6 in newborn heterozygous (+/−) and homozygous (−/−) Cx50S50P and Cx50G22R mutant lens. (A) Levels of protein derived from the spliced XBP1 message (Xbp1(s)) are apparently increased in the both heterozygous mutants although western blotting did not detect consistent differences in levels in homozygous mutants. (B) ATF4 levels are moderately higher in Cx50 S50P mutants and while no changes were seen for the Cx50 G22R mutant as compared to wildtype. (C) Atf6 protein levels are slightly higher in Cx50 S50P mutant lenses while no consistent changes were detected for Cx50 G22R mutants as compared to wildtype.