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. Author manuscript; available in PMC: 2013 Nov 20.
Published in final edited form as: Clin Chim Acta. 2012 Jul 6;413(23-24):1839–1847. doi: 10.1016/j.cca.2012.06.034

Figure 1.

Figure 1

Multiple reaction monitoring ion chromatograms for quantification transitions: m/z 519–343 (11-nor-9-carboxy-Δ9-tetrahydrocannabinol-glucuronide: THCCOOH-glucuronide), m/z 489–313 (Δ9-tetrahydrocannabinol-glucuronide: THC-glucuronide), m/z 343–245 (11-nor-9-carboxy-Δ9-tetrahydrocannabinol: THCCOOH), m/z 329–268 (11-hydroxy- Δ9-tetrahydrocannabinol: 11-OH-THC), m/z 313–245 (cannabidiol), m/z 311-223 (cannabinol) and m/z 315–193 (Δ9-tetrahydrocannabinol: THC). Panels A–G are extracted blank human urine, panels H–N blank human urine fortified with analytes at the limit of quantification and panels O–U are from an authentic urine specimen collected 23 h after smoking a 6.8% THC (w/w) cigarette. Limits of quantification are 0.5 ng/ml for THC-glucuronide, 1 ng/ml for 11-OH-THC, THCCOOH and cannabidiol, 2 ng/ml for THC and cannabinol and 5 ng/ml for THCCOOH-glucuronide. Urine cannabinoid concentrations in panels O–Q are 213, 2.9 and 15.1 ng/ml of THCCOOH-glucuronide, THC-glucuronide and THCCOOH, respectively. Concentrations for all analytes shown in panels R–U were less than limits of quantification. Qualifier transitions are not displayed for sake of clarity, but are listed in Table 1.