Table 1.

Liquid chromatography tandem mass spectrometry parameters for cannabinoids and cannabinoid glucuronides in human urine.

Analyte	Q1	Q3	Dwell	Declustering	Entrance	Collision	Collision	Cell exit	Retention
	mass	mass	time	potential	potential	entrance	energy	potential	time
	(amu)	(amu)	(msec)	(volts)	(volts)	potential	(volts)	(volts)	(min)
						(volts)
THC	315.3	193.2	50	51	4.5	16	29	4	6.23
	315.3	123.2	50	51	4.5	16	43	4
THC-d3	318.3	196.2	50	51	4.0	14	31	4	6.22
	318.3	123.0	50	51	4.0	14	43	4
11-OH-THC	329.1	268.1	50	−50	−4.5	−14	−46	−2	5.24
	329.1	311.2	50	−50	−4.5	−14	−28	−4
11-OH-THC-d3	332.2	271.2	100	−50	−5.0	−18	−42	−4	5.23
	332.2	314.1	100	−50	−5.0	−18	−26	−4
THCCOOH	343.1	245.0	100	−60	−5.0	−16	−40	−2	4.13
	343.1	190.9	100	−60	−5.0	−16	−42	−2
THCCOOH-d9	352.2	253.9	50	−55	−8.5	−18	−42	−4	4.10
	352.2	194.3	50	−55	−8.5	−18	−44	−4
Cannabidiol	313.2	245.2	25	−55	−5.5	−14	−32	−2	5.78
	313.2	179.2	25	−55	−5.5	−14	−28	−2
Cannabidiol-d3	316.2	247.8	25	−55	−6.0	−14	−34	−2	5.77
	316.2	182.2	25	−55	−6.0	−14	−26	−2
Cannabinol	311.3	223.2	100	46	4.0	18	29	4	6.18
	311.3	178.1	100	46	4.0	18	79	4
THC-glucuronide	489.2	313.2	100	−60	−4.5	−24	−44	−4	3.11
	489.2	112.9	100	−60	−4.5	−24	−30	−2
THCCOOH- glucuronide	519.3	343.2	50	−50	−4.5	−24	−32	−4	3.10
	519.3	299.2	50	−50	−4.5	−24	−46	−4

Q1= quadrupole 1, Q3= quadrupole 3, THC= Δ9-tetrahydrocannabinol, 11-OH-THC= 11-hydroxy-THC, THCCOOH= 11-nor-9-carboxy-THC.

Bold masses depict quantification transitions.