Table 2.
Mean extraction efficiency and matrix effect for cannabinoids and cannabinoid glucuronides extracted from urine by supported-liquid extraction
| Analyte | Extraction efficiency | Matrix effect | ||||
|---|---|---|---|---|---|---|
| (%, N = 4) |
(% of signal suppressed, N = 4) |
|||||
| Low | Mid | High | Low | Mid | High | |
| THC a | 38.4 | 33.9 | 41.1 | 28.0 | 16.8 | 4.8 |
| 11-OH-THC b | 58.6 | 50.0 | 48.0 | 5.6 | 14.9 | 17.8 |
| THCCOOH b | 71.2 | 69.8 | 67.6 | −0.8 | −1.6 | −2.4 |
| Cannabidiol b | 53.8 | 40.8 | 43.9 | −10.3 | 8.9 | −0.7 |
| Cannabinol a | 40.9 | 35.6 | 41.4 | 16.4 | 13.4 | 7.6 |
| THC-glucuronide c | 71.4 | 72.6 | 69.6 | 31.8 | 4.8 | −1.9 |
| THCCOOH-glucuronide d | 65.5 | 64.4 | 61.2 | 88.7 | 24.1 | 7.1 |
| THC-d3 | 40.8 | 35.2 | 41.5 | 27.5 | 12.0 | 3.6 |
| 11-OH-THC-d3 | 54.8 | 49.9 | 47.7 | 6.8 | 15.4 | 14.5 |
| THCCOOH-d9 | 69.9 | 67.9 | 65.1 | 4.3 | 1.8 | 1.3 |
| cannabidiol-d3 | 52.6 | 41.7 | 44.7 | −7.3 | 12.0 | −1.7 |
THC= Δ9-tetrahydrocannabinol, 11-OH-THC= 11-hydroxy-THC, THCCOOH= 11-nor-9-carboxy-THC.
THC and cannabinol low, mid and high quality control concentrations were 6, 25 and 75 ng/ml, respectively.
11-OH-THC, cannabidiol and THCCOOH low, mid and high quality control concentrations were 2.5, 25 and 75 ng/ml, respectively.
THC-glucuronide low, mid and high quality control concentrations were 1.5, 15 and 45 ng/ml, respectively.
THCCOOH-glucuronide low, mid and high quality control concentrations were 7.5, 75 and 225 ng/ml, respectively.
THC-d3, 11-OH-THC-d3, THCCOOH-d9 and cannabidiol-d3 concentrations were 10 ng/ml.