Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Nat Neurosci. 2012 Sep 2;15(10):1414–1421. doi: 10.1038/nn.3209

Figure 3.

Figure 3

Calcium currents during the pacemaking cycle in DMV neurons. (a) cobalt-sensitive calcium currents recorded (bottom) in response to a voltage clamped spike waveform recorded from a DMV neuron (top). Inset: distribution of the cobalt-sensitive subthreshold calcium current measured prior to the spike. (b) population average (mean ± sem) of the persistent cobalt-sensitive calcium currents as a function of voltage. Solid red line is a fit as in Fig. 2c, but with a calcium Nernst potential of +120 mV. The fit for the TTX sensitive current from Fig. 2c is shown in black for comparison. (a) single-cell reverse transcription PCR (sc-RT-PCR) reveals the presence of mRNA for Cav1.2 and Cav1.3 channels in DMV cholinergic neurons (in 7/8 and 3/8 cells, respectively). (d) cobalt-sensitive calcium action currents generated by a physiological spike (red) and one expanded to twice the duration (blue). Vertical dashed line is the time of spike threshold, as determined by maximal dV/dt. Inset: calcium charge (=shaded area) as a function of spike duration.