(b)

PATS typea	PCR amplification and restriction digestion patterns of amplicons obtained using 7 PATS primer pairsd							Isolatesc
	IKNR3	IKNR7	IKNR10	IKNR12	IKNR16	IKNR27	IKNR33
1	2	2	2	2	2	2	2	5, 168, 817, 908, 928, 977, 1015, 1492
2	2	2	2	0	2	2	2	268, 757, 806, 807
3	2	2	2	2	1	2	2	1041
4	0	2	2	2	1	2	2	827, 5671, 5974
5	1	2	2	2	1	2	0	214, 528, 961, 1016, 1273, 1286, 1328
6	2	1	1	2	1	2	2	425, 935

^aPATS types are designated arbitrarily with different numbers.

^bPrefixes of each PATS primer pair A/B are indicated. 0: no amplicon; 2: amplicon with one XbaI site.

^cBovine E. coli O157:H7 isolates from different locations that fell within a given PATS type.

^dPrefixes of each PATS primer pair A/B are indicated. 0: no amplicon; 1: amplicon without AvrII site; 2: amplicon with one AvrII site.