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. 2012 Sep 20;2012:936518. doi: 10.1155/2012/936518

Figure 1.

Figure 1

Expression of TRPV4 mRNA and protein in HCD-cells. (a) and (c) represent RT-PCR analysis using primers specific for human TRPV4 and SGK, respectively. PCR products of 618 bp and 699 bp were observed in three RNA preparations (1, 2, and 3) corresponding to mRNA expression for TRPV4 and SGK in HCD cells. Negative controls included both water and samples in which AMV enzyme had been omitted from the reverse transcription reaction (data not shown). Western blot analyses of HCD cell lysates ((b) and (d) 5 μg protein/lane) using an antibody against human TRPV4 and SGK confirmed the presence of the protein. A protein band of approximately 120 KDa (TRPV4) and 50 KDa was detected. Controls included TRPV4 and SGK antibody preabsorbed with a 100-fold excess of immunizing peptide (data not shown).