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. Author manuscript; available in PMC: 2012 Sep 30.
Published in final edited form as: Biochemistry. 2010 Nov 2;49(43):9269–9279. doi: 10.1021/bi101102u

Figure 1.

Figure 1

FlK confers fluoroacetate resistance in vivo. The viability of E. coli BL21(DE3) was assayed by spotting 1:10 serial dilutions of culture onto solid media in the absence (left) or presence (right) of 20 mM fluoroacetate. E. coli harbored no plasmid (top row), empty pET23a-His10-Tev (second row), pET23a-His10-Tev-FlK (third row), or pET23a-His10-Tev-FlK-H76A (fourth row).