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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Int J Biochem Cell Biol. 2012 Jul 21;44(11):1942–1951. doi: 10.1016/j.biocel.2012.07.016

Figure 1.

Figure 1

Identification of RecQL4 nuclear export signals by GFP-GFP system. (A) Schematic representation of GFP-GFP-RecQL4_NESs. All of putative NESs in RecQL4 were examined by NES consensus: φ-X(2,3)-φ-X(2,3)-φ-X-φ, φ= L,I,V,F,M; X=any amino acid. The key hydrophobic amino acids were boxed. c-Abl-NES was used as positive control; (B) Subcellular distribution of GFP after transient transfection of GFP-GFP-NES constructs (ABL1-NES, pNES1–3 & pNES5) in U2OS cells; (C) Percentage of cells with more GFP protein in nuclear than in cytoplasm (N>C) or more in cytoplasm than in nuclear (N<C). A total of 200 GFP-positive cells were counted in each of three independent experiments.