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(A) An increased mtDNA copy number in HEK293 cells after an induced expression of wild type RecQL4, not RecQL4 ΔNES2&3 in the presence of 0.5µg/ml of doxycycline for 48 h; (B) Analysis of mtDNA copy number in U2OS cells after shRNA-mediated RecQL4 suppression. The mtDNA copy number was quantified by real-time quantitative PCR by comparing the ratio of mtDNA and nDNA. *, p<0.05; **, p<0.01.
