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m-S100A9- and lipopolysaccharide (LPS) -induced responses are Toll-like receptor 4 (TLR4) -dependent. Bone marrow-derived dendritic cells (BM-DC) from C57BL/6, TLR4-knockout (KO) and RAGE-KO mice were stimulated with 100 ng/ml LPS or 20 μg/ml m-S100A9 for 48 hr. Secretion of cytokines (a) and nitric oxide (NO) (b) was determined in culture supernatants. Data of three independent experiments were compiled and analysed using Student's t-test: *P < 0·05; ***P < 0·005.
