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. 2012 Jun 18;12:28. doi: 10.1186/1475-2867-12-28

Figure 7.

Figure 7

Knocking-down expression of Kaiso in K562 cells reduced monocyte-macrophage differentiation. Flow cytometric measurement of granulocytic specific surface antigen CD15 and CD11b in K562 cells after normalization with the scrambled knock-down expression. K562 cells were transfected with the indicated siRNA combinations A, B and C. The CD117 (c-Kit receptor), CD33 and the erythroid markers GPA and CD71 were also analyzed. (D) CD15 expression in K562 knockdown cells. M1 represents unlabeled K562 scrambled control. M2 represents K562 scrambled control labeled with CD15 and M3 represents K562 cells transfected with Kaiso duplex (10 nM) after 50 h-incubation. Data were expressed as mean ± standard deviation (S.D.). Columns, mean (n = 3); error bars, S.D.; *, p < 0.001.