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Cotranscriptional loading of proteins onto growing BR pre-mRNA molecules. Some proteins bind to the pre-mRNA with high sequence specificity (e.g., CBP20), whereas others bind with lower specificity along the entire RNA molecule (e.g., the SR protein hrp45 and the 2xRBD-Gly protein hrp36). The RNP fibril formed serves as the substrate for trans-acting factors, and its structure affects a number of mRNA-related processes, including splicing, transport, and translation.
