Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 1;199(1):77–95. doi: 10.1083/jcb.201206033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Marzi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — DA-7 miRNAs cooperatively inhibit DNA replication and accelerate the onset of terminal differentiation. (A) DA-7 miRNAs were transfected at low doses (1 nM) alone or in combination in cycling C2C12 myoblasts, and the effect on proliferation was evaluated using the Delfia cell proliferation kit. Bars show the mean and standard deviation over control (SCR, scrambled oligo) in two independent experiments. The asterisk marks a significant (P < 0.05) p-value by Student’s t test. (B) The effect of DA-7 overexpression (1 nM) was analyzed by RT-qPCR (24 h after transfection) on several proliferation and differentiation genes. (C) Myotube (C2C12) formation was assessed upon transfection of DA-7 miRNAs at low doses (1 nM) in a 3-d differentiation assay. Differentiation was followed by visual inspection by phase-contrast microscopy. Enlarged/hypertrophic muscle fibers are indicated with arrowheads. (D) At day 1, multinucleated myotubes (>2 nuclei) were counted after immunostaining with MyHC antibody. Insets show a 1.5× magnification. Bars: (C) 20 µm; (D) 100 µm.