Figure 5.

Overexpression and suppression of PsbA2R. A, The sense and antisense fragments of PsbA2R were fused to the rnpB promoter, yielding overexpressor [PsbA2R(+)] and suppressor [PsbA2R(−)], respectively. The control ΔpsbA3 strain contains only the kanamycin (Km) resistance cassette. B, Segregation check of transformants. Disruption of the psbA3 gene and replacement of the slr2031 region were checked by PCR. WT, Wild type. C, Expression of PsbA2R and psbA2 mRNA in the overexpressor [PsbA2R(+)] and suppressor [PsbA2R(−)] mutant strains, respectively. One and 5 μg of total RNA isolated from the cells grown under GL were used for the detection of psbA2 mRNA and PsbA2R, respectively.