Figure 9.

Comparison of osmotic-stress- and ABA-related gene expressions between the wild type (Col-0) and tt2-5 under stressed environments. Total RNA was isolated from the whole seedling 10 DAS. RT-PCR was performed with osmotic-stress- and ABA-related gene primers. The cDNA samples were normalized by RT-PCR using a tubulin probe as an internal control. A, 1× MS agar medium without stress. B, 1× MS agar medium containing 3% (w/v) Glc. C, 1× MS agar medium containing 100 mm NaCl. D, 1× MS agar medium containing 1 μm ABA. Boxes of solid lines indicate an up-regulation of a gene in the tt2-5 mutant; boxes of dashed lines show a down-regulation of a gene in the tt2-5 mutant.